Sentence examples for multiple cassettes from inspiring English sources

We found that this reduced activity was due to recombination of the expression cassette repeat sequences during the transduction of the lentiviral vector, which resulted in deletions of one or multiple cassettes.

Induction of recombination using a beta-cell-specific and tamoxifen-inducible line, Tg ins:Cre-ER T2 ), can switch the red fluorescence of beta-bow to either green or blue in multiple cassettes within one genomic integration site (Supplementary Fig. 1B), producing a color palette that can specifically label beta-cells in distinct color combinations.

Multiple cassettes were most efficiently amplified using Pfu with an annealing temperature of 66°C, more than 15°C higher than suggested by the manufacturer.

Cassette PCR has shown its effectiveness in recovering single and multiple cassettes (including interspersed attC sites) in the hands of Stokes and colleagues [15] and others [16], and by us in an analysis of cassette diversity from sites in and around Halifax Harbour [17].

While specific chromosomal integration sites are more difficult to identify, non-homologous integration allows multiple cassettes to be incorporated, a common phenomenon in H. jecorina [ 27, 28].

As a consequence, integrons are normally associated with arrays comprising multiple cassettes, which in some species of Vibrio can number well in excess of a hundred [ 7].

It comes with multiple cassette designs that resemble popular blank tapes of the era, including a bright red style that is reminiscent of West German rock and roll cassettes circa 1989, perfect for recreating the heady days of the Velvet Revolution.

Several other strategies developed for multiple cassette cloning in different situations were reviewed and compared [7], [14] [16].

Previous studies focus on the regulation of a single cassette exon, but recent experiments indicate that multiple cassette exons within a gene may interact with each other.

Additionally, there were two insertion sites; namely, tRNA-Pro-GGG and the TCA cycle isocitrate dehydrogenase gene, icdA, where several small genomic regions were inserted in a multiple cassette fashion for several genomes, indicating insertion loci of genomic plasticity (Table  3, Table  4).

All this can be significantly time-consuming and multiple-cassette containing strains may be unstable and difficult to maintain, losing copies of the inserted cassettes over time [ 14].