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Fortunately, Cetacea has an extensive fossil record (see [ 32]) permitting the use of multiple calibration constraints.
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The use of multiple calibrations as constraints on the nodes of interest has the advantage of using empirical data to constrain solutions.
Maximum (U) and minimum (L) time constraints in million years ago (Ma) at nodes depicted in Additional file 1 The MCMCTREE program allows for minimum (lower) and maximum (upper) time constraints, and multiple calibration points have been argued to provide overall more realistic divergence time estimates [ 70].
The MCMCTREE program allows for minimum (lower) and maximum (upper) time constraints, and it has been argued that multiple calibration points provide more realistic divergence time estimates overall [91].
The multidivtime program allows for both minimum (lower) and maximum (upper) time constraints and it has been argued that multiple calibration points would provide overall more realistic divergence time estimates.
Multiple calibration points were used in BEAST analyses.
Local clock models were recently extended to incorporate multiple calibration points and multiple genes [7].
Calibration constraints (in Myr) used as priors in the BEAST analysis of cytochrome b of soricids.
Calibration constraints (in Myr) used as priors in the BEAST analysis of mammalian introns.
To exclude the possibility that individual calibration constraints may bias our dating analyses, we repeated them after removing each calibration point in turn following [ 16].
Moreover, use of relaxed molecular clocks and fossil calibration constraints also permits estimation of divergence times of clades.
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