However, EBV infection in whole multiple sclerosis brain sections or single cells isolated from the CSF was very rarely detected (Morré et al., 2001; Willis et al., 2009; Peferoen et al., 2010; Sargsyan et al., 2010).
In a recent report, Serafini et al. (2010) were unable to detect LMP2A and EBNA1 transcripts by quantitative real-time polymerase chain reaction in multiple sclerosis whole brain sections.
FoxP2 expression in area X of multiple coronal or sagittal brain sections was quantified from digitized images of film autoradiograms using Adobe Photoshop 7.0 (Adobe Systems Inc. San Jose, CA) as previously detailed [14].
To obtain full images of brain sections, multiple grey-scale images were acquired per section using Spot Advanced software (Spot Imaging Solutions) and merged into a full image and inverted into black-on-white images using Photoshop CS4 (Adobe Systems, San Jose, CA).
For each antibody, at least 4 AD and 4 WT sections from multiple brain regions were assessed.
In both cases, basophilic to achromatic NCIs were readily identified on H&E or cresyl violet stained sections involving multiple brain regions including the primary motor cortex, ventral spinal cord, inferior olive, dentate nucleus of the cerebellum, basis pontis, substantia nigra and globus pallidus.
Furthermore, follow-up staining of sagittal brain sections from P28 wild-type (C57BL/6J) and Mecp2-null (Mecp2tm1Bird/y) mice confirmed significantly decreased EGR2 in multiple areas of the brain, including medulla, cerebellum and pons (Supplementary Material, Fig. S7).
In sagittal brain sections from several independent strains of mice harboring the K14-eGFP transgene, eGFP was detectable in multiple regions of the brain with the highest level usually found in the forebrain and cerebellum (Supplementary Material, Fig. S3).
Is it a matter of multiple brain processes happening together?
