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To determine if DP-called genes are biologically significant, one needs to use multiple biological replicates per condition (Hansen et al., 2011).
The time-course experiments were repeated several times to provide multiple biological replicates for microarray analysis.
Screens designed to identify factors that can increase insulin expression should therefore be adequately powered and possess multiple biological replicates.
At this stage, all remaining peptide identifications were screened for presence in multiple biological replicates and those proteins identified by two peptides were subject to manual validation for final confirmation.
This provides a robust and powerful dataset with low variance due to a combination of stringent experimental protocols and multiple biological replicates and its statistical strengths make possible sophisticated bioinformatic analyses and network predictions [55], [73].
Thus, multiple biological replicates for nRIP are required.
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Multiple independent biological replicates, each containing a pool of twenty different plants, were sampled for mRNA-Seq (two biological replicates) and reverse transcription-PCR (RT-PCR) or quantitative RT-PCR (qRT-PCR) validation (three biological replicates).
Besides displaying expression profile from multiple biological or technical replicates within an experiment, the average expression of replicates can also be generated when checking "Show Average" checkbox.
Microarray quality was validated by the good reproducibility of experimental measurements of multiple biological and analytical replicates.
Expression levels of all statistically significant and differentially expressed mRNAs and miRNAs were confirmed by qRT-PCR using TaqMan® expression kits (Applied Biosystems) [65] using multiple technical and biological replicates.
This is demonstrated in the next section, where we used real biological data with multiple biological and technical replicates.
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multiple biological modules
multiple experimental replicates
multiple biological redundancies
multiple biological functions
multiple biological scales
multiple biological processes
multiple biological targets
multiple biological consequences
multiple biological samples
multiple biological fluids
multiple biological factors
multiple biological barriers
multiple biological effects
multiple technical replicates
multiple biological activities
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