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We defined NOVA CLIP tag clusters by grouping overlapping RNA tags identified in multiple biologic replicates (biologic complexity (BC) > 2) (Licatalosi et al., 2008) from four independent animals.
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Thus, multiple biological replicates for nRIP are required.
Error bars represent the SEM for multiple biological replicates.
Reproducibility in multiple biological replicates is not discussed.
Shown are representative images present in multiple biological replicates.
All experiments were performed multiple times by using independent biologic replicates.
In both the biologic replicates, the median log ratio of 1 was considered for expression analysis.
Subject 3 had the most number of previous treatments including multiple systemic treatments and multiple biologic therapies.
Such cLBP is often termed nonspecific and may be due to multiple biologic and behavioral etiologies.
The critical role of hedgehog signaling in multiple biologic processes raises concerns about its potential therapeutic use in cancer.
These biologic replicates were pooled.
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