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Gene expression profiling was performed in multiple batches using Affymetrix U133A microarrays.
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These reads were assembled in three batches using Velvet/Oases and a multiple- k-mer approach (see Materials and Methods).
RNA extraction and gene expression profiling were performed in multiple batches over time using Affymetrix U133A microarrays.
Working in batches, use a blender to purée soup.
Binding experiments were carried out in triplicate for each protein peptide combination, and checked using multiple batches of protein.
Experiments were repeated using multiple batches of labeled RNA, with both forward and reverse-labeling to account for dye bias, for a total of 16 two-colour arrays.
This has been a consistent finding in our laboratory using multiple batches of recombinant protein (both CKD and FL) and with both primary monocytes and the monocytic cell line THP-1.
To improve the credibility of the results, multiple batches of SBP and RBP should be used.
Electron microscopy of multiple batches of the silver citrate colloid using a Zeiss Supra 40 VP field-emission gun scanning electron microscope (FEG-SEM; Carl Zeiss SMT GmBH, Oberkochen, Germany) operating at a voltage of 1 kV established that we had produced nanoparticles of 70 ± 30 nm (data not shown).
For SSRPs with a very short half-life (O water and N ammonia) a long transfer line may be used to deliver multiple batches to a remote area for further processing.
When brewing multiple batches of beer, be sure to use water that was heated when it passed through the heat exchanger to replenish the hot liquor tank.
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