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Many currently recognized species contained multiple barcode lineages, often with deep divergence suggesting unrecognized species.
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As a result of our processing, sequences that were shorter than 200-nt or had multiple barcode or primer motifs were excluded from the analysis.
This study involved the Astraptes fulgerator (Walch, 1775) species complex and found ten distinct DNA barcode lineages.
For example, the fall armyworm, Spodoptera frugiperda, includes two barcode lineages with 1.3percentnt divergence (figure 2 c).
Barcode sequencing can be facilitated by converting individual Sfi I site-bordered barcodes to barcode oligomers, which allows for the generation of multiple barcode sequences per Sanger sequencing reaction, providing a cost-effective and rapid alternative compared to cloning and sequencing individual barcodes.
Here, we investigate three DNA barcode lineages of what has been identified as Urbanus belli (Hesperiidae, Eudaminae) in Área de Conservación Guanacaste (ACG), northwestern Costa Rica.
Reads matching equally to multiple barcodes were discarded.
If wsp PCR bands were present in some or all individuals of a DNA barcode lineage, all specimens from that lineage underwent the more extensive Multilocus Sequence Typing (MLST) assay [ 18] to identify the specific strain of Wolbachia.
Finally, peripheral blood DNA is derived from multiple cell lineages.
CD44High/CD24Low cells were able to self-renew and give rise to multiple cell lineages.
They can be induced into multiple cell lineages under certain conditions.
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