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PCR results showing multiple bands indicated male mice, which were then used in this study.
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Native-PAGE results showed that multiple bands indicating purification were not successful (data not shown).
In addition, the multiple bands indicate a mix of various patterns of methylated CpG sequences.
Northern blots of 6S (sCAC1377) and S-box (SAM, sCAC1132) revealed multiple bands indicating possible post-transcriptional processing by enzymes such as RNaseP, as has been reported [ 44].
The resulting bands were separated in a 0.8% agarose gel to test for the presence of single or multiple bands (indicating the absence/presence of insertions deletions in the molecule).
Multiple-bands indicated by uppercase a in Table 2) generally occur only in specific organs in both cultivars as in CITSI24 CX077363, CITSI47 3, CITSI57 1 and CITSI41 1.
No clear evidence of film debonding was found, which, together with the observation of multiple shear bands, indicated good adhesion and ductility of the metallic-glass film.
Interestingly, palmitoylated CD86-HA was observed as multiple high MW bands indicating that its glycosylated forms are palmitoylated [ 43], while its non-glycosylated form at the expected molecular weight of 35 kDa was not modified, potentially suggesting sequential modification.
OsGLP12+C′GFP showed two bands indicating multiple isoforms.
The 26S probe usually hybridised to several high molecular weight bands indicating the presence multiple IGS families lacking 5S insertions.
Deglycosylation decreased the intensities of the higher molecular weight bands and increased those of the lower molecular weight bands, indicating that the multiple bands are different glycoforms rather than different fragments of EpCAM.
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