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Indeed, western blots on human PC3, HeLa, and NTera2D1 cell lysates indicate the presence of multiple bands corresponding to the above computer predictions (Figure 5b).
Since SUMO-1 was used in the assay mixtures, the observation of multiple bands corresponding to sumoylated CBS in the vitro assay (Figure 1A), which has also been reported previously [8], and to CSE (Figure 4) suggests that sumoylation occurred at more than one site.
Infections with more than one strain of P. jirovecii, which occur frequently in HIV-infected patients (20%70%% of patients) can be easily detected with this method if the different strains have a different number of repeats, since PCR amplification will result in multiple bands corresponding to the different sizes of the repeats.
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Wild-type embryos revealed multiple bands, the largest (~210 kDa) corresponding to full-length Scrib protein (Fig. 5D).
Only sample AF/CDC5 showed bands corresponding to the multiple bands of the Sm complex.
Given the multiple bands appearing on the p38 immunoblots, the specific band corresponding to phospho-p38 was identified by comparison to the positive control.
Multiple size selections were performed using electrophoresis, and bands corresponding to sizes of 200, 300, 400, and 600 bp were excised and purified from a single continuous DNA smear.
Indeed, with this antibody, we observed multiple etoposide-induced bands by western blot analysis, including bands corresponding to VCP phosphorylated at Ser784 (97 kDa) and Chk2 phosphorylated at Thr26/Ser28 (62 kDa).
As expected, blots from mock-infected cells contained various bands corresponding to the 3 main isoforms of full-length APP in multiple glycosylation states [32] (Figure 1B).
δEF1 has multiple consensus sites for phosphorylation by Erk kinases (Costantino et al, 2002), and the bands corresponding to δEF1 by SDS PAGE migrated faster after in vitro dephosphorylation by alkaline phosphatase (see top right panel in Supplementary Figure S3E).
The bands corresponding to acrosin I and II were observed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com