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Single particle analysis by AFM elucidated that NLP-1 was comprised of numerous different sized NLP species (Figure 2C), corroborating the presence of multiple bands by NDGGE.
PCR products were purified either directly or, in cases of multiple bands, by cutting bands from 1% agarose gels and using the QIAGEN spin system.
Three more papers in the same issue provided Western blotting data revealing that antibodies to muscarinic and adrenergic receptors (Jensen et al. 2009) gave multiple bands by Western blotting in KO tissue.
The PEX5 N-terminal is sensitive to proteolysis [23,35 37] and during pull down experiments some degradation occurred which was detected as multiple bands by the anti-PEX5 antibody raised against the N-terminal region [29] (Figs. 3 and 4, anti-PEX5 panel).
Of 52 membrane proteins examined, 27 revealed the expected cell-specific cell-surface expression pattern and 25 antibodies failed due to absence of signal in all assays tested or were detected as multiple bands by western blot (see Supplemental Experimental Procedures for antibody details).
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The multiple bands detected by RT-PCR and Western blot correspond to different PAX8 isoforms previously described [ 18].
Zfy1-b transcripts were amplified with primers in exons 8 and 10, to avoid the multiple bands generated by PCR from exon 1b for Zfy1.
However, we noticed that there were multiple bands observed by western blotting with higher molecular weights of approximately 130 kDa when U2OS cells were synchronized at mitotic phase, and there were fewer bands with lower molecular weights during progression through mitotic exit.
Although multiple bands were recognized by the anti-FLAG antibody in both cell lines, the specific band identified by the anti-AR antibody was only shown in the AR cells.
All asRNAs detected were long (0.5 8 kb) but notably heterogeneous in size, with multiple bands being visible by northern hybridization.
Isoforms and degredation products typically result in multiple bands when separated by SDS-PAGE; however, western blots of the prostate lysates reveal a single 80kD OPH band.
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