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They fail to: (i) account for multiple bacterial subpopulations with differing susceptibilities; (ii) quantify or interpret the explicit interaction (synergy/antagonism) mechanisms; and (iii) accommodate spontaneous mutations.
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Proportions of bacterial subpopulations (intracellular, replicating extracellular and non-replicating extracellular) before and during treatment with multiple regimens.
Alternatively, LasR proficient bacterial subpopulations might have been present in the initial infection but these subpopulations were not identified in our study.
This demonstrates that DX expression disrupts bacterial growth in multiple bacterial strains, which suggests that DX is targeting a conserved bacterial pathway.
Selective media were used to study the bacterial subpopulations [ 9].
Loss or gain of genes within bacterial populations may indicate niche adaption of bacterial subpopulations [ 23].
Genes that are differentially maintained in bacterial subpopulations are likely to reveal differential selective pressures acting on these subpopulations [ 20].
While tobacco exposure might favor a bacterial subpopulation-associated pathway leading perhaps to inflammation, alcohol exposure may be agnostic to bacterial subpopulations or induce carcinogenesis independent of specific microbial subpopulations.
These bacterial subpopulations may contribute to different aspects of disease pathogenesis.
Bacterial subpopulations significantly differentiated tobacco users from non-users (P < 0.008) (Fig. 5A).
Three patients sustained multiple bacterial infections.
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