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Close paralogues, particularly when tandemly arrayed, and subtle differences in the structure of gene models across multiple assemblies of the same genome sequence present significant challenges.
Using various mixtures of read coverages from paired-end and linear 454 libraries, multiple assemblies of varied quality were generated.
The initial assembly of pooled 454 BAC sequences showed 40% inflation compared with estimated insert sizes, which likely was caused by multiple assemblies of repetitive sequences.
> This co-evolutionary clustering reveals multiple assemblies of genes that have clear modular functional association (see Supplementary File S3 for the complete list of clusters).
These were derived from multiple assemblies of 1.3 billion RNA-Seq reads, selected for uniqueness and putative protein-coding quality, and represented samplings from mixed sources of vegetative and reproductive organs, seedlings, embryos, haploid megagametophytes, and needles under environmental stress (Supporting Information, File S1).
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Multiple assemblies and annotations of the bovine genome exist from different sources (e.g. Ensembl, NCBI, UCSC, UMD).
This is an elegant combination of multiple assembly processes; assembly of tetramers in solution, through-membrane dimerization and inclusion of dendrimers into these membrane-bound channels.
We used the subtractive multiple- k method of Surget-Groba and Montoya-Burgos (2010) and constructed multiple assemblies using a range of k-mer lengths (25, 29, 33, 37, 41, 45, 49, 53, 57, 61, 65, and 69).
It achieves this by using an array of hash lengths, a dynamic filtering of noise, a robust resolution of alternative splicing events and the efficient merging of multiple assemblies.
To quantify the assembly quality in relation to the different variables of our experiment, we devised measures that enable comparisons of multiple assemblies.
Our work extends previously developed algorithms for de novo and comparative assembly, enabling integration of multiple assemblies at once.
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