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We analyzed the enhancer trap collection for lifespan, selecting ten lines that had significantly increased longevity during multiple assays in both males and females.
Multiple assays in triplicate on the pG5-luc transfected clones yielded induction levels ranging from 32- to 51-fold for the four best Panc02+RHeoSwitch-3 clones and from 7- to 94-fold for the four best Panc02+RHeoSwitch-4 clones.
Multiple assays in triplicate on the GAL4-SEAP transfected clones gave induction levels ranging from 118- to 551-fold for the best five Panc02+RHeoSwitch-3 clones and from 52- to 486-fold for the best twelve Panc02+RHeoSwitch-4 clones.
These results are statistically highly significant and repeated in multiple assays in multiple strain backgrounds carried out over a period of six months.
Notwithstanding this ambiguity, SMF reproduced cellular effects of the A2AR antagonist AM241385 in multiple assays in PC12 cells in the current study.
Therefore, an approach of multiple assays in plant antioxidant potential screening work is highly desirable [ 10].
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This is an interesting and informative paper on the concept of PAINS (pan-assay interference compounds), compounds that have apparent activity in multiple assays, but in fact produce false signals due to non-specific interactions or interference with the assays.
Of the chemicals that had AC50/LEC values < 1 μM in multiple assays, some showed cytotoxicity in one or more of the cell-based assays, which suggests cytotoxicity pathway activation, although in many cases we do not have a specific (cell-free) assay that would indicate which pathway that was.
Multiple assays were performed in approximately 5 min at different flow rates, using membranes saturated with varying antibody concentrations.
The rats were given drinking water with or without the cytokine inhibitor pentoxifylline (PTX) starting the day before fracture and continuing for 4 weeks, after which time the cast was removed and multiple assays were performed in the hindpaw.
Many of the compounds in the present study were biologically tested in multiple assays, and hence, a substantial fraction of conformer pairs appear in multiple assays.
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