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Many of the compounds in the present study were biologically tested in multiple assays, and hence, a substantial fraction of conformer pairs appear in multiple assays.
In order to understand the effects of autophagy in a given biological settings, it is absolutely necessary to carry out multiple assays and compare the results of these investigations as a whole.
To support multiple assays and high throughput on the array, a four-chamber hyb cap was used so that four different concentrations of Ag could be applied on a single array; and, within each chamber, Ppy deposition was controlled on 50 blocks of 4 electrodes with respect to deposition time and voltage or current.
In addition to lowering the amount of DNA required for multiple assays and, therefore, requiring fewer redraws for patients, it also allows for very specific amplicon placement.
Together, we used multiple assays and reproducibly found that BTB could effectively inhibit the ER-positive MCF-7, Ishikawa, and SKOV-3 cell growth.
Using multiple assays and different muscles with different fiber type compositions, our data are consistent and show muscle weakness, pathological changes, and gene expression changes.
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Monitoring of multiple assay and test sample variables showed no significant long term trends or degradation in any of the critical assay reagents or reportable values for both assays.
A second screening was performed with 11 S. aureus isolates to demonstrate utility of the Gene-Z card for testing multiple assays simultaneously and for comparing with culture-based susceptibility.
Stem-loop reverse transcription PCR (RT-PCR), which can be applied in small RNA cloning and multiple assays for better specificity and efficiency, was used for reverse transcription [ 86].
The effect of ART on cell death induction was tested in multiple assays, including viability assays and the cytotoxicity assay.
Complementary information from multiple assays, pharmacokinetic modeling and mechanistic follow-up studies are necessary components of the overall framework.
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