Furthermore, the modular design of the device accommodates a multiplex aptamer selection protocol.
To perform the multiple target aptamer selection, we developed a workflow process wherein a set of microcolumns, each prefilled with a different target-immobilized resin, are arranged into a serial configuration.
In the development of sensitive, selective molecular markers for CRC, cell-based aptamer selection holds significant promise by its potential to identify multiple useful markers in a relatively short time.
The method for nucleic acid aptamer selection is quite simple and yields highly specific aptamers within a short time [ 4, 6].
We chose allosteric NAE as the basis of our sensor development program simply because NAE is able to convert one binding event to multiple signals during catalytic turnovers, and it could be utilized as a platform to conduct aptamer selection [26] [32].
Although SELEX has proven to generate specific aptamers for several targets, MonoLEX can be a valuable alternative for aptamer selection.
To select DNA aptamers specific for Vaccinia virus (VACV) as a model for OPV, aptamer selection was performed in two steps.
Therefore a modified oligonucleotide pool is becoming more popular for aptamer selection.
Variations of the aptamer selection strategies can be used to isolate catalytic nucleic acid sequences by changing the binding selection step of the aptamer selection process to an activity selection step (Fig. 15).
Here, we summarize key recent developments in aptamer selection methods, as well as other aspects of aptamer selection that have significant impact on the outcome.
