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Many transcripts have multiple antisense transcripts in plant.
One transcript may form NATs with multiple antisense transcripts [ 15].
A transcript may form more than one trans-NAT with multiple antisense transcripts.
The large genomic sequence sizes of Glyma13g11820.1 (78,178 bp) and Glyma13g11940.1 (101,408 bp) may help to explain the reason they contained multiple antisense transcripts.
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If this confirmation was extended to other in silico predictions of antisense transcription it would support the existence of multiple natural antisense transcripts (NATs) in U. maydis.
Of the 8,634 transcripts that form NATs, 3,846 contain multiple (2 177) antisense transcripts.
Our analyses [14] and those of others [117], [118] establish by multiple independent criteria that Arabidopsis antisense transcripts are real and of biological significance.
More than half of these unique transcript contigs contain multiple sequences of either splice variants or antisense transcripts.
For um02794, a gene that had multiple ESTs supporting the existence of a sense and antisense transcripts, RT-PCR using strand-specific primers for first strand synthesis detected both transcripts in haploid cells grown in two nutritional conditions.
Multiple cells with RNA foci composed of sense or antisense transcripts were detected, and these were most often observed in proximity to the Purkinje cell layer separating the granular and molecular layers (Fig. 4c).
While most of the published transcriptome analyses in P. falciparum do not permit differentiation between sense and antisense transcripts, some studies provided a strand-specific analysis and revealed antisense transcription from multiple sites across the genome [ 7, 15, 16].
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