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The multiple anchor primers allowed multiple positions to be sequenced with the same set of query.
Multiple anchor primers that were identical except that each progressive primer was shorter by one nucleotide were used.
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In an ATDOA system, there are multiple anchor Tx nodes and one anchor Rx node.
Furthermore, the anchor point of known Halpern type iteration is single, however our iterative sequence has multiple anchor points.
3' RACE PCR reactions were carried out using gene specific primers and anchor primers supplied with the kit.
We used a single anchor; our results require validation with alternative anchors or multiple anchor methods.
Nested PCR was performed using two so2426-specific primers (2426-GSP2: 5'-TGCACTAACCGTCGCTCTATGGCCTGCAAA-3' and 2426-GSP3: 5'-GCACCAAGTTCATAGCTTCGTATCCTGTCT-3') and the manufacturer-supplied abridged anchor and abridged universal anchor primers.
The anchor primers (Anchor Primer, see Table 1) were designed to hybridize onto the 5' end of the Test Template, and provide a free 5' phosphate to ligate the query primers (Extension Primers, see Table 1).
A saturation step was performed to fully saturate all Anchor Primers sites not extended during the Query Primer ligation cycle.
Anchor primers were designed within the Pitx2ab, Pitx2c and Enpep promoter sequences.
The DNA was then amplified in the reaction using the two types of anchor primers.
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