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Using the gene hierarchy allows more fine-grained annotation, with multiple alternate transcripts, CDSs, UTRs, etc.
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Defective mRNA splicing can lead to multiple alternative transcripts including those with retained introns, cassette exons, alternate promoters/terminators, extended or truncated exons, and reduced exons.
Thus, starting from lists of the most differentially expressed genes within a trait, the multiple gene partners, alternate transcripts, functionally similar genes, conserved structural motifs or sequences, and any other quantifiable aspect of a protein or gene region can be added, allowing for the construction of a gene network relevant to the underlying disorder in question.
The current study provides evidence for four alternate transcripts of FVIII in the endothelium.
The nature of the smaller band is not known as small alternate transcripts of DAAM1 are not reported.
Two alternate transcripts were observed in the RT-PCR assays.
However, no alternate transcripts were detected at the RNA level.
We show that dogs do express alternate transcripts in the brain utilising an alternate exon homologous to human exon 28.
For variants containing multiple alternate alleles, we separated each alternate allele as one unique variant and normalized them independently.
Bioinformatic analyses including identification of antisense transcripts, alternate transcripts, and TFs were conducted as previously described [ 43].
Additional file 10: Schematic of the guanylate binding protein 5(gene )gene and alternate transcripts.
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