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We conducted phylogenetic footprinting by several alternative methods: generalized Lempel-Ziv complexity (C LZ ), multiple alignments with DIALIGN and ALIGN-M, and the MOTIF SAMPLER Gibbs sampling algorithm.
Multiple alignments with Probalign retained 491 reliably aligned genes from a set of the 497 orthologous genes.
ClustalW [31] was used for multiple alignments with manual refinement.
Here we use standard multiple alignments with substitutions and indels on 21 flu virus genomes (figure 4).
Multiple alignments with complete sequences or domains were conducted using the CLUSTALW program [68] using default parameters and then manually revised.
Multiple alignments with other reported l-nLDHs showed that all catalytically important residues (Arg 109, Asp 168, Arg 171, and His 195) were conserved in the B. coagulans SDM l-nLDH (Figure S1) [27].
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The CDV H gene sequence was aligned with all available H gene sequences in GenBank using Multiple Alignment with Fas Fourier Transform (MAFFT) with default settings [ 32].
Sequence alignment was done in BioEdit (version 7.0.9.0) using ClustalW Multiple Alignment with default settings.
Conceptual translation of the ORFs into amino acid sequence was done to compute a multiple alignment with ClustalW [64].
Research into multiple alignment with rearrangements has been limited, although some progress has been made [9], [28] [31].
A multiple alignment with the 48 predicted Aco ORFs was carried out with Clustal_X [ 47].
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