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The resulting alignment was aligned to the six PUR repeats by submitting both multiple alignments to HHalign on the Bioinformatics toolkit.
We also discerned those markers uniquely aligned to A, B and C genomes, and those markers with multiple alignments to different genomes, respectively, which were used for the population genetic analysis for the diverse lines of the three A genome-carrying species.
An individual read may produce multiple alignments to one or more references.
The locations were refined using multiple alignments to other crustacean nucleotide sequences.
The range of the multiple alignments to be included in the analysis was decided based upon alignment quality.
If a read had multiple alignments to the same amplicon, the entire region spanning across multiple alignments was kept.
Similar(26)
These sequences were aligned using Clustal Multiple Alignment to identify conserved and variable regions suitable for primers and probes that could differentiate the species.
DBL5ε homology models were created by submitting the multiple alignment to the HHpred server [38].
In order for a multiple alignment to be meaningful in this context, all sequences in the multiple alignment must have a common origin.
Therefore, it becomes more efficient to restrict motif searching or multiple alignment to this set of promoters.
Most phylogeny [20], [21], community distance estimates [11], [22], and abundance distributions [23] [25] ultimately rely on input from a multiple alignment to compute sequence distances within a consistent alignment template.
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