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Some existing clustering software calculates distances from multiple alignments that may be sub-optimal.
In the third pass we use the resulting, highly specific read overlap sets to construct multiple alignments that will be used to correct errors.
Protein weight matrix Gonnet, with Gap Opening 10 and Gap Extension 0.2 was used for multiple alignments that were manually refined in GeneDoc v.2.7.0 [34].
The multiple alignments that did not yield cassette estimations may be dispersed gene repeats or have a larger cassette than our parameters allowed (400 bp on each side).
Our new pipeline consists of multiple alignments that incorporate all known genetic variants into a genomic model followed by annotation and merging.
Two of these are datasets of multiple alignments that contain information on a single individual per species (D s and D s, 0 ).
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Primers were designed on a multiple alignment that included all known LCDV genotypes.
Thus far, manual curation of a megabase-scale whole-genome multiple alignment that includes rearrangement and lateral gene transfer has proven too time-consuming and difficult.
Mismatches were observed in several Hymenoptera taxa included in this multiple alignment that affected all of the stems.
The compatibility criterion detected breakpoints by identifying neighbouring sites in the multiple alignment that gave incompatible phylogenetic signals.
Any nucleotide position in the multiple alignment that failed to have information for at least three samples of the alignment (4.47% of the samples) was deleted on the grounds that it was unlikely to represent homology shared across the alignment.
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