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In addition, copies (4 50) in each species (supplementary table S1, Supplementary Material online) were also aligned using MUSCLE, and their consensus sequences were reconstructed using the above multiple alignments in each genome using DAMBE (Xia and Xie 2001) after gaps were removed.
These blast results identified possible frame shifts and intron inclusions because they appear as multiple alignments in the Blast output.
Mugsy computed the multiple alignments in <1 h (37 min) after completing the pairwise searches with Nucmer.
We validated the effectiveness of this approach by empirically generating multiple alignments in which motif sites were lost with a frequency determined by our exponential loss function.
All contigs were aligned with the set of original reads from which they were assembled, generating multiple alignments in ACE format.
The accuracy of reconciliation methods depends on the quality of initial phylogenetic data, usually gene trees, and multiple alignments in selected cases.
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Using this new subroutine for aligning alignments, we can then perform multiple alignment in RAF using a standard progressive strategy (Feng and Doolittle, 1987).
Conservation analysis of SEP3 (A) and SEP12 (B) with clustal multiple alignment in six species.
Conversely, poor performance is seen when multiple alignment in not reliable or completely unavailable.
In order to identify segmental duplications, we plotted the phylogeny (neighbor joining, 1000 bootstraps) next to the multiple alignment in eBioX [17] (summarized in Fig. 5).
This will find the best sequence in DatabaseA for the profile made from a multiple alignment in DatabaseB.
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