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A similar observation was made by ContextMap (Bonfert et al., 2012) that multiple alignments from a RNA-seq aligner could be handled by removing the incorrect alignments based on the 'context' of the alignments.
The multiple alignments from the five structural motifs in actin were plotted using WebLogo [18].
For each read Rr we once again construct multiple alignments from the new set of reads in Rr.
However, no BLAST alignment exceeded 2kb in length, nor did multiple alignments from the same hit approximate the full-length insertion sequence, although the 118 kb unmapped scaffold AAAB01008935 bears three consecutive islands of similarity corresponding to ∼7 kb of the insertion sequence.
We used the EM algorithm to estimate ncRNA substitution rates from two datasets: (1) a subset of multiple alignments from release 7 of RFAM [37] whose annotated secondary structure was derived from a published source; (2) a set of pairwise alignments derived from the mix80 dataset used to parameterize the CONSAN program [38], which in turn was derived from the European Ribosomal RNA database.
Multiple alignments from significant local pair wise alignments.
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The consensus multiple alignment from the six procedures was made by M-Coffee [ 46] implemented in the T-Coffee package.
We have randomly split each multiple alignment from each database into two subalignments and thus obtained two sets of multiple alignments (lets us call them DatabaseA and DatabaseB).
Figure 10 c is an example multiple alignment from which the profile HMM given in Fig. 10 b could be learnt.
If the best sequence found in Database B belongs to the multiple alignment, from which we started the matching procedure, then this multiple alignment belongs to the set of BBHs.
Striving to more accurately define phylogenetic relationships in the P. furiosus, P. horikoshii, P. abyssi and T. onnurineus species group, we used the concatenated multiple alignment from the 911 orthologous clusters.
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