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The twenty-one GBSSI sequences were compared by multiple alignments considering the Nipponbare sequence as the reference.
The ClustalW program [ 55] and the alignment editor BioEdit [ 56] were used to generate multiple alignments considering the collected plant GSTs.
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To extend the usefulness of contact prediction, we have designed a new meta-predictor (MetaPSICOV) which combines three distinct approaches for inferring covariation signals from multiple sequence alignments, considers a broad range of other sequence-derived features and, uniquely, a range of metrics which describe both the local and global quality of the input multiple sequence alignment.
The sequence alignment between hTAS2R38 and the eight structural templates were extracted from the multiple sequence alignment considering the entire family.
Wheeler & Giribet [ 77] made clear their judgment on multiple alignments and considered it a non-scientific procedure.
Only regions that overlap the reference match by at least one base pair in the original multiple alignment are considered in the realignment.
Each position of the heavy and the light chain multiple alignment was considered as a variable; therefore, we had 135 variables for the heavy chain and 125 variables for the light chain.
The one-to-one relationship can be used, among other things, in deciding which multiple alignments should be considered for detection of possible mutations, or even detection of possible sequencing errors.
Multiple alignments have been considered a computational rather than a biological issue and much more effort has been employed in improving algorithms for matching individual nucleotides under a similarity criteria than to address what evidence must be pursued for aligning nucleotides that share evolutionary descent (see Morrison [ 74] for a revision).
In a standard RNA-seq pipeline, the 'best' alignment for a fragment with multiple alignments is determined without considering the surrounding alignments of other fragments.
In the GeneScissors pipeline, we first collect all possible alignments for all fragments, and then examine those regions of the genome where multiple alignments map and then consider the other fragments aligned to these regions.
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