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We focus on the regulatory regions of multiple alignments by ignoring the non-conserved regions.
Protein identity was calculated based on multiple alignments by using CLUSTALW [ 58].
This move prohibits the emergence of multiple alignments by similarity of multiple short CNBs to a single long CNB.
As a control, for each CNE we also generated 1000 multiple alignments by randomly shuffling the columns of each alignment using the seqboot implementation in Phylip version 3.67.
Structured novel ncRNAs were predicted from the multiple alignments by RNAz version 2 pre-release based on structure conservation in between species [ 80].
Homologs between H. Sapiens and S. cerevisiae were obtained by similarity measures from the whole-genome multiple alignments by the Ensembl comparative genomics team.
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One can also make adjustment to the multiple alignment by hand.
Alignment of each retrieved orthologous data among all strains under investigation was performed in AMAP (Protein multiple alignment by sequence annealing) [ 37] with 0.5 as the gap factor.
For this comparison, a single set of LCBs was first calculated by Mugsy to define genomic extents for multiple alignment by MLAGAN, Pecan and FSA.
Then nucleotide sequences of the references near each SNP and the reads of meiotic products nearby were selected for detailed multiple alignment by CLUSTALW [ 33].
That is, it reduces the computational costs of constructing a multiple alignment by reducing this task to a sequence of pairwise alignments of profiles representing previously calculated multiple alignments of subsets of the original sequence set.
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