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Multiple alignments between BnaABF2 and seven other ABF proteins revealed a high level of sequence similarity among each other (Fig. 1).
Multiple alignments between PtrABF and eight other ABF proteins revealed high sequence identity among each other.
Indeed this is expected; protein-protein searches also report multiple alignments between pairs of proteins.
Multiple alignments between Aq.AphC.like proteins and prokaryotic AphC are shown in Figure 1.
In a second step, multiple alignments between all 22 passerine sequences were obtained by MUSCLE (codon based).
Moreover, multiple alignments between the rag locus and Sus showed that the RagA and SusC were both constituted of three domains (TonB I, II, and III); and RagB and SusD were constituted of two domains: Lipoprotein and RagB/SusD, respectively.
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To measure secondary structure conservation beyond rodents and hominids, we obtained a multiple alignment between mouse genome assembly mm7 (NCBI Build 35) and fifteen other vertebrate genomes from the UCSC Genome Browser Database [64].
Next, we executed multiple alignment between the retrocopies and their parental gene sequences using ClustalW2 (Larkin et al. 2007).
A multiple alignment between a known site and all sites included in the corresponding profile was generated and the alignment score was estimated as described above.
However, Figure 4B shows a multiple alignment between spumaretroviruses and errantiviruses, which according to the qualitative domain similarity merits further attention.
Each iteration in the algorithm involves two steps: (i) Given the current alignment, build an optimal CM and (ii) given the current CM model, find the optimal multiple alignment between the sequences.
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