Sentence examples for multiple alignment were from inspiring English sources

The evolutionary genetic distances (expressed in terms of the number of differences per 100 bases or amino acids, including length polymorphisms) between every pair of sequences in a multiple alignment were generated using the DISTMAT program accessible at http://hpc.ilri.cgiar.org/emboss/ [36].

Thus, all sequences in the resulted multiple alignment were less than 65% identical with each other.

Nonconserved regions in each multiple alignment were removed using an in-house script.

Values for multiple alignment were gap opening penalty 15, gap extension penalty 0.3, and delay divergent sequences 25%.

A few manual adjustments to the multiple alignment were made before running Gblocks, which yielded 1,679 positions.

After gap removal all families with less than 25% or more than 98% identical nucleotide positions in the multiple alignment were filtered out.

The goal of multiple alignment is to align sequences according to their evolutionary relationships.

For each DNA sequence, a multiple alignment was created by Clustal W Thompsonn et al. 1994).

Multiple alignment was constructed with CLUSTALW [26].

A multiple alignment was achieved within each GH family.

Multiple alignment was done with ClustalX2 under default settings.