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Multiple alignment was created by aligning all sequences against Hidden Markov Model GTP_EFTU from Pfam [ 105] using program HMMALIGN [ 18].
DsrC-like predicted protein sequences were aligned with Muscle v3.8.31 (Edgar, 2004), and the multiple alignment was displayed with Jalview (Waterhouse et al., 2009).
Amino acid multiple alignment was checked using Gblocks [ 72] and ambiguously aligned sites were removed.
Multiple alignment was done with the Phylogeny Inference Package (PHYLIP version 3.5c, J. Felsenstein and the University of Washington, Seattle, WA).
Sixteen metagenomic MCP sequences were aligned with the MCP sequences from complete virophage genomes, and the resulting multiple alignment was used for phylogenetic tree construction.
For each DNA sequence, a multiple alignment was created by Clustal W Thompsonn et al. 1994).
Multiple alignment was constructed with CLUSTALW [26].
A multiple alignment was achieved within each GH family.
Multiple alignment was done with ClustalX2 under default settings.
18 placental mammals from the Multiz 28-way multiple alignment was used as input.
A statistical In silico analysis of the multiple alignment was performed using the SigniSite server [23].
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