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Based on maximum identity score and query coverage, the best highly identical 10 sequences were selected and aligned using multiple alignment software program, ClustalW (MEGA tool).
On the basis of maximum identity score, the sequences were selected and aligned by using a multiple alignment software program such as Clustal W. Distance matrix was generated using RDP database and the phylogenetic tree was constructed using MEGA 5 (Tamura et al. 2013).
All sequences were aligned using Muscle, a multiple alignment software for protein and nucleotide sequences which allows multiple sequence comparison by log-expectation [38].
COI and 28S sequences were aligned using the web interface of the multiple alignment software MAFFT ([33]; available at http://www.ebi.ac.uk/Tools/mafft/index.html), under default settings.
The identified BVMO sequences were combined with the known bacterial BVMOs sequences and analyzed using CLUSTALW multiple alignment software (http://www.ebi.ac.uk/Tools/msa/clustalw2/). Further sequence analysis based on consensus motifs was made.
Sequence alignments were created using Muscle multiple alignment software (58).
Similar(44)
Multiple sequence alignments of deduced amino acid sequences were made using the ClustalW multiple-alignment software (http://www.ebi.ac.uk/clustalw/index.html).html
The quality of the 16S rRNA gene sequences was checked using the multiple alignment CLUSTALW software package [ 37].
PCR products were sequenced by de-deoxy sequencing and sequences were aligned using Multi-Align [37], a multiple sequence alignment software available online (http://bioinfo.genotoul.fr/multalin/multalin.html).html
The amino acid sequences of PKR, PKZ and PERK kinase domains (see Table 2 for accession numbers) were aligned using MUSCLE, a multiple sequence alignment software [ 64], and the resulting alignments were checked using MacClade (Sinauer Associates).
Comparisons of operational taxonomic units (OTUs) based on V9 domains and (almost) complete SSU rRNA sequences are almost impossible on large data sets of sequences because none of the multiple sequence alignment software is able to properly align SSU rRNA sequences within their divergent domains, and this problem is exacerbated for short divergent tag sequences (Guillou & Christen unpublished).
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