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Exact(8)
With the multiple alignment results of human, mouse and rat on UCSC Genome Browser, we used the 100 bp upstream and downstream sequences of all the putative binding sites as the input sequences of PAML [55] to get their phylogenetic tree.
Conserved sequences of the multiple alignment results were shaded using the multiple-alignment printing and shading program, BOXSHADE 3.21.
Multiple alignment results show that in this specific location only one species (in Mus musculus) has variation which is Proline.
The multiple alignment results indicate a high degree of structural conservation, as the alignment is primarily comprised of a small number of large LCBs common to multiple genomes.
CNS coordinates and sequences are provided in supplementary material files, Supplementary Material online, in FASTA format, and also multiple alignment results in supplementary material files, Supplementary Material online.
Based on the multiple alignment results, CFs were extracted from genes with alignment scores > 50, longer than 30 nt, and limited to one fragment per nonoverlapping gene.
Similar(52)
All Drosophila, Apis, Anopheles, and partial lepidopteran nAChR subunit protein sequences were aligned with ClustalX and the multiple alignment result was used as input into the PAUP software (v.4.0b10) to construct phylogenetic trees with the maximum parsimony method and bootstrapping sampled for 1,000 times [ 31], and the tree was viewed with TreeView application [ 32].
The multiple alignment result clearly shows the highly conserved DBD domains among pepper Hsf genes.
The multiple alignment result also shows that lktA sequences from O and A1 are more similar to one another and most of the variations identified by three-way comparisons are due to the lktA sequence of the B genome.
An additional 1322 SNPs were determined to be within exons however discrepancies of SNP positions across multiple alignments resulted in uncertainty of codon position.
The multiple alignments resulted in an unrooted distance tree using neighbour-joining algorithms of MEGA version 5. The reliability of the tree was examined using bootstrap analyses (1000 replicates).
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