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The multiple alignment representation was accomplished with Jalview [ 8].
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We agree that the improvement in contig lengths mainly stems from the multiple alignment with consensus representation of the components, which seems to be a more appropriate output for contigs belonging to different subspecies due to variations.
Nucleotide sequences were translated to protein using ExPASy translate (http://web.expasy.org/translate/) The sequence alignment was done using ClustalW2 multiple alignment (http://www.ebi.ac.uk/Tools/msa/clustalw2/) and representation was done using BoxShade Server (http://www.ch.embnet.org/software/BOX_form.html) which highlight identities and similarities in protein sequence.
For each such column (in the multiple alignment), we transform it into a vector representation m : S → ∑ A A ∪ ′ - ′, ′ ?
A graphical representation (sequence logo) of the multiple alignment of these repeats is shown in Fig. 4B.
To assign columns to clusters, we weight sequences in a multiple alignment to eliminate any distortions in frequency vectors that could be caused by unequal representation of similar sequences in a multiple alignment.
An algorithm for progressive multiple alignment of sequences with insertions.
(c) Multiple alignment of the NREP ORF across vertebrates.
Multiple alignment analysis was performed using Multalin (http://multalin.toulouse.inra.fr/).inra.fr/
It then constructs a multiple alignment that is maximally consistent with the improved pairwise alignments.
multiple alignment using fast Fourier transform.
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