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To optimize the multiple alignment, proteins were truncated at the domain boundaries of the CA3427 sequence.
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M-Coffee V9.0 program [ 65] was used to perform the multiple alignment of proteins in each family.
Amino acid sequence corresponding to predicted genes was extracted and used for multiple alignment with proteins belonging to other baculoviruses.
Multiple alignment of proteins sequences and phylogenetic analysis revealed that SlIPT3 and SlIPT4 are closely related to Arabidopsis ortholog AtIPT3 and AtIPT5, as previously reported [ 12].
The main difficulty is in constructing a meaningful multiple alignment with proteins whose number of zinc fingers varies from 7 to 21.
Multiple alignment of protein sequences was performed by MUSCLE [ 100] and then manually edited.
Multiple alignment of protein sequences and visualization of the detected post-translational modification sites were performed using CLC Sequence Viewer (CLC Bio).
To test our method, we used standard benchmark databases for multiple alignment of protein and nucleic-acid sequences.
The sequence translation, exon organization, the multiple alignment and protein signature sequences have been analysed with Biology workbench.
Since each orthologous group is represented by a multiple alignment of protein sequences, we need a very fast algorithm to compare multiple alignments.
Additionally, evolution at the level of amino acid sequence was calculated from a multiple alignment of protein sequences of the persistent genes.
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