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Two different multiple alignment programs were then used to align the single read and the genomic sequences: "multalin" and "clustalw".
CLUSTALW multiple alignment programs (http://www.ddbj.nig.ac.jp and http://mobyle.pasteur.fr) were used to align the amino acid sequences.
For each of these multiple alignment programs, we used the matches returned by BLASTER and then clustered by GROUPER, RECON or PILER.
This comparative study (Table S3) shows that all the multiple alignment programs gave similar results in terms of sensitivity and specificity, whether launched after GROUPER or after PILER.
The latest versions of 8 different multiple alignment programs (see below) were used to construct an alignment for each of the benchmark test sets.
POY may share drawbacks with other automated multiple alignment programs.
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For phylogenetic analyses, the sequences were aligned using multiple alignment program CLUSTAL_X [ 43].
For phylogenetic analyses of each of these 10 species data sets, the sequences from each of them were assembled and aligned using the multiple alignment program CLUSTALW version 1.82 [1.82.
All proteins were aligned using the multiple alignment program CLUSTALX with default settings [ 18].
The mitochondrial sequences were aligned using the multiple alignment program of BioEdit [ 65] and manually checked and edited.
For phylogenetic analyses, rrs sequences of each of these 15 species were assembled and aligned using the multiple alignment program - ClustalX version 2.0.12 [ 73].
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