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A rough solution may involve first applying multiple alignment procedures to these sequences and then segmenting the aligned sequences into subsequences involving different numbers of species followed by segment-wise Monte Carlo integration.
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The multiple alignment procedure is a modification of the pair-wise superimposition method outlined above.
We can also take these different multiple alignments and cluster them in order to see how the OTU abundance results depend on the multiple alignment procedure.
The input for our multiple alignment procedure is a set of supergene core sequences belonging to one orthologous group obtained at the previous step.
At Göttingen Bioinformatics Compute Server (GOBICS), we provide a user-friendly web interface where anchor points can be used to guide the multiple alignment procedure [ 21].
We adopted the following strategy: We used information provided by DSSP (Kabsch and Sander, 1983) on beta partners (BP) and we only considered "complete" strands (undamaged by the DSSP assignment and the FSSP automatic multiple alignment procedure).
As expected from this multiple alignment procedure, the resulting phylogenies outside the lophiiforms are identical to those reported in Yamanoue et al. [ 27] and the order Lophiiformes is confidently recovered as a monophyletic group with 100% bootstrap probabilities (BPs) in all datasets.
Using multiple alignments procedures, conserved or substitutive amino acid [ 42] patterns were chosen, in each set or subset of proteins, without discriminating between functional and non-functional regions.
This comparison is achieved using standard multiple alignment benchmarking procedures.
Mückstein et al. [ 15] suggest the use of the method as a starting point for improved multiple sequence alignment procedures.
Yet serious difficulties exist in proposing reliable hypotheses of nucleotide homology for regions of ambiguous alignment (RAAs) using standard multiple sequence alignment procedures.
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