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The goal of multiple alignment is to align sequences according to their evolutionary relationships.
Multiple alignment is the starting point of almost all analyses performed on microbiome sequences.
Multiple alignment is more accurate than a series of pair-wise alignments and this allows for Sn to be extended to include additional fully conserved residues.
A multiple alignment is built for each cluster using the representative and up to 30 of its nearest sequences with ClustalW [39].
The complete linkage clustering with our hand curated multiple alignment is found to be robust with respect to choice of clustering radius.
Multiple alignment is usually the first pipeline step.
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For each DNA sequence, a multiple alignment was created by Clustal W Thompsonn et al. 1994).
Multiple alignment was constructed with CLUSTALW [26].
A multiple alignment was achieved within each GH family.
Multiple alignment was done with ClustalX2 under default settings.
18 placental mammals from the Multiz 28-way multiple alignment was used as input.
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