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We name "work positions" positions in the multiple alignment for which at least 8 amino acids are aligned.
After assembly, all reads of origin were aligned against belonging contigs and metacontigs, obtaining a multiple alignment for each of them.
An iterative alignment refinement strategy as described in Tóth et al.48 was employed: maximum-likelihood gene trees computed from preliminary alignments (using RAxML, see below) were used as guide trees for the next round of multiple alignment for PRANK.
In cases of more than one multiple alignment for a given human region (e.g., with different indels), we selected the one with the best alignment score.
Distance estimation using our proposed finite-state transducers (blue and green lines) came close to the performance of classical multiple alignment for closely related species.
Irrespective of evidence for positive selection, in the multiple alignment for each cluster, individual residues at each position were examined for polymorphism that correlated to tissue-specificity.
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Castresana, J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis.
Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis.
The result of the multiple alignments for amino acid sequences is shown in Figure 1.
However, exploring enough multiple alignments for large datasets is prohibitively expensive and therefore remains impractical for now.
Although IDE preference for cleavage is after aromatic and hydrophobic residues, multiple alignments for substrate binding can occur [46].
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