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Poorly aligned residues within amino (N)- and carboxyl (C)- termini and within predicted loop/turn regions were trimmed from MSA to improve downstream bioinformatics analyses using the multiple alignment editing program Jalview [ 40].
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The resultant 15,500 base pair (bp) multiple alignment was edited by hand to minimize the number of unique gaps and to ensure the integrity of the reading frame (available online as Figure S1).
The ClustalW Multiple alignment and manual editing were performed using BioEdit Sequence Alignment Editor (Ibis Biosciences, Carlsbad, CA).
Multiple alignments were edited with the SEAVIEW program [ 52] and used in the phylogenetic analyses.
The sequences were subjected to multiple sequence alignments using GCG (pileup) or ClustalW, and multiple alignments were edited using Boxshade software.
The multiple alignment therefore is edited [ 10] in Cys/Gly-rich regions to favor showing Cys-Gly-Gly as the common inserted or deleted tripeptide.
However, the original program had many limitations only one multiple alignment could be edited at a time, and an alignment's colouring and tree-based conservation analysis could only be exported as a figure, not stored and returned to later.
Indeed, the strong reduction in the searching space that was obtained due to the application of TUIUIU, and the ability of our filter to deal with large error rates, allowed the tool to perform better also at the qualitative level due to the removal of sequences that were not candidates for best local multiple alignment with bounded edit distance.
A manually edited multiple alignment was drawn (available at http://biosrv.cab.unina.it/citrusGST/).it/citrusGST/
The mitochondrial sequences were aligned using the multiple alignment program of BioEdit [ 65] and manually checked and edited.
The authors took advantage of available structures to guide editing of the multiple alignment, which in turn allowed them to associate conserved positions with the structural features.
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