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All multiple sequence alignments were carried out using MAFFT [ 85] and visualized using Multiple align show (http://www.bioinformatics.org/sms/multi_align.html).html
Using the protein sequences for the AP2, HSF, SEN1 and non-symbiotic hemoglobin encoding genes in rice and Arabidopsis, multiple sequence alignments were carried out using MAFFT [ 77] and visualized using Multiple align show http://www.bioinformatics.org/sms/multi_align.html.html
The BioEdit and Clustal W programs were used to multiple align individual sequences with the reference sequence (GenBank accession number: NT008583 or March, 2006 assembly hg18: chg18:71599909-71602173).
The sequences of Excalibur and 11 other cultivars were aligned to Psy-A1t allele sequence of the breeding line WAWHT2074 [GenBank:HM006895] [ 9] and Psy-A1s allele sequence of the cultivar Schomburgk [GenBank:EU649795] [ 8] with the multiple align module implemented in Geneious® 6.0.4 (Biomatters Ltd., New Zealand) with default settings.
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In alignment-based mode, the same motif can occur at multiple aligned positions within a single family; in that case only the highest BLS value is used.
Supplementary Figure 5 Multiple aligned pre-and postsynaptic modules represent discrete synaptic profiles within individual spines.
Amino acid sequences were multiple aligned through CLUSTAL-W software and compared for conserved sequence motifs.
The 16S rRNA sequences of isolates were multiple aligned using the ClustalW algorithm.
Moreover, fabricating a device with multiple aligned NWs using the aforementioned method is quite difficult.
a The nucleotide sequences of plastid trnM (cau) gene from nine species of seed plants were multiple aligned by using LaserGene (DNAstar, USA).
They were multiple aligned using ClustalW [52] in Mega 3.1 Alignment Explorer with default parameters.
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