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The present study documented both multiple aggregates and typical perinuclear localized aggresomes in HeLa cells over-expressing the C-terminal truncated αA-crystallin genes.
Given that a single VLP structure can split into multiple aggregates and dimers, the potential for increasing the total antigen concentration is large.
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There are 4 basic types of fruits: simple, multiple, aggregate and accessory.
In order to force immediate formation of a more compact pellet, avoid the generation of multiple aggregates from a single well, and increase throughput, we employed 96- and 384-well V-bottom plates.
In the 16X5 and 9X5 simulations, multiple aggregates were initially formed in the membrane, which subsequently coalesced together to form the larger aggregate.
Then the capillary rise from the saturation pan into the multiple aggregates (set of 14 or 15 aggregates) without and with coatings was measured.
Hierarchical EZ-AG addresses this issue by providing each node with multiple aggregates of neighborhoods of increasing size around itself.
Aggresomes are special protective structures that fundamentally differ from other multiple aggregates, that some of which cause cellular toxicity.
Interestingly, when cells contained multiple aggregates, these aggregates were always distributed to only one of the daughter cells, whereas the other daughter cell was free of any aggregate.
It has been reported that multiple aggregates or pre-aggresome particles may be an intermediate step in aggresome formation which can proceed further upon inhibition of proteasome [33].
As pointed out by the reviewer, Sup35 often forms multiple aggregates in [ PSI+] cells.
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