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On the basis of the amplification results, the nucleotide sequences of three representative homoeologous microsatellites were amplified from multiple accessions of T. urartu and T. monococcum and compared.
The fact that many genetically dissimilar varieties can share the same seed characters (such as red color or small grains) may help explain why multiple accessions of the same name were found to be genetically different.
Of nine species, six of them such as O. rufipogon (AA), O. nivara (AA), O. glumaepatula (AA), O. glaberrima (AA), O. sativa (AA), and O. punctata (BB) contained both SUB1B- and SUB1C-like genes side by side on chromosome 9 (Chr 9), as shown in Fig. 1a and b, which is consistent with the observations in multiple accessions of O. sativa (AA) (Singh et al., 2010; Xu et al., 2006).
This feature of the AFLP technology can be exploited to expedite polymorphism discovery by generating in parallel highly similar genome representations of multiple accessions of crop species for high-throughput sequencing.
Numbers after species names indicate that multiple accessions of that species were included.
For these latter analyses, multiple accessions of the same species were pruned by keeping the most early-branching accession.
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Sequencing multiple accession of Epipogium allowed us to assess the level of intraspecific plastome polymorphism the first intraspecific comparison of complete plastid genome sequences from nonphotosynthetic plants.
We therefore generated 18S and matR sequences from multiple accessions and species of Apodanthaceae from the United States, Chile, Iran, and Australia.
Results for a number of the intron-containing species, including B. jamaicensis, were confirmed by sequencing multiple accessions from each of these species (Additional File 1).
In the absence of whole-genome draft sequences, high-throughput sequencing of genome representations of multiple accessions in parallel using CRoPS will supply sufficient genetic (single nucleotide) polymorphisms to allow marker-assisted selection using existing genotyping platforms.
Our study of complete plastid genomes of multiple accessions in M. truncatula revealed a significant intraspecies ptDNA variation.
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