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Multilocus sequence testing comparison with porcine strains may clarify whether transmission is from pigs/porcine products or of human-adapted, H. suis-like, bacteria.
For example, our understanding of methicillin-resistant Staphylococcus aureus (MRSA) dissemination in humans and animals depends on the use of genotypic methods such as multilocus sequence testing to identify outbreaks of disease (Kock et al. 2009) and to detect otherwise unrecognized disease clusters (Mellmann et al. 2006).
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Isolates with spa types associated with ST398 were confirmed by multilocus sequence typing; tested for Panton-Valentine leukocidin toxin, mecA, and nuc genes; and typed for SCC mec as described (6 – 9 ).
In this paper, we use multilocus sequence data to test whether A. lucens attacking Galerucella larvae represent one generalist or multiple specialist species.
Multilocus sequence type (MLST) testing was performed as described by King et al. (24 ), with a modification for mutS as described by Rehm et al (25 ).
Antimicrobial drug susceptibility testing, multilocus sequence typing (MLST), and eBURST analyses were performed as described (13 ).
Seventy-five isolates were submitted to antimicrobial susceptibility testing, multilocus sequence typing (MLST) and polymerase chain reaction (PCR) for SCC mec, accessory gene regulator (agr) groups, arginine catabolic mobile element (ACME) and Panton Valentine leukocidin (PVL) toxin genes.
If a woman has the same serovar present at follow up, further testing by multilocus sequence typing (MLST) targeting ompA and five house-keeping genes will be undertaken [ 28, 29].
ESBL-producers were further characterized by multilocus sequence typing (MLST) and antimicrobial susceptibility testing using broth microdilution.
To test this hypothesis, we used multilocus sequence typing (MLST) to analyze a large collection of 250 isolates of these four O serogroups, which were isolated from diseased as well as healthy humans and cattle between 1952 and 2009.
Swabs were tested for S. aureus, and isolates were tested for antimicrobial susceptibility and subjected to multilocus sequence typing.
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