DNA sequencing methods, including whole genome sequencing and multilocus sequence analysis (MLSA), likewise have proved useful in the identification of bacteria.
multilocus sequence analysis.
Multilocus sequence analysis (MLSA) attributed both isolates to the S. pneumoniae group.
Multilocus sequence analysis and MLST gene fragments were repeatedly inspected when getting the whole genome nucleotide sequences of strains (see below).
Recently, one of the housekeeping genes, gyrB, has been used as an alternative target in multilocus sequence analysis (MLSA) to provide greater phylogenetic resolution.
We therefore decided to investigate the possibility of constructing a molecular epidemiology tool based on multilocus sequence analysis (MLSA) with PCR amplification of various loci followed by sequencing.
Further diversification of species within each of these two evolutionary branches of Leptospira is supported by multilocus sequence analysis and DNA renaturation kinetics [3], [14], [15].
Even though there are phenotypic differences between the isolates, there is not a consistent signal in the multilocus sequence analysis that allows one to differentiate the biovars.
This study shows that multilocus sequence analysis and comparative genomic hybridization based on a mixed genome array is a powerful method for studying species assignment within the E. cloacae complex.
Multilocus sequence typing (MLST) [27] or multilocus sequence analysis (MLSA) (the latter refers to genus-wide analyses) [28] based on housekeeping genes are considered to be the most powerful genotyping tools in studies of the population biology of microbial organisms.
Sequencing of six or more genes from different loci allows a multilocus sequence analysis (MLSA) that can define bacterial species without sequencing a whole genome or performing DNA-DNA hybridizations [29].
