Phylogenetic trees for these taxon sets, based on multilocus sequence analyses (MLSA; [ 26- 28]) are shown in Figure 1, with and without the reference taxon as indicated.
Based on multilocus sequence analyses, however, these isolates are A. viridinutans, a mold phenotypically resembling but phylogenetically distinct from A. fumigatus.
The relationships of V. cholerae have been studied in several ways, but the most useful insights have come from multilocus enzyme electrophoresis (12 ) and more recently by multilocus sequence analyses (4, 13, 14 ).
During the preparation of this manuscript a new L. taiwanensis species isolated from silage was described [ 39]. 16S rRNA encoding gene and multilocus sequence analyses showed that our strains isolated from rat intestine belong to L. taiwanensis species.
Subsequently, multilocus sequence analyses were performed and targeted the internal transcribed spacer (ITS) 1 and 2 regions flanking 5.8S rDNA, (partial) β-tubulin gene (benA), and (partial) calmodulin gene as previously described (1 – 4 ).
To begin to understand the underlying genetics that complicate species level discrimination, we compared the genomes of Harveyi clade members isolated from different environments (seawater, shrimp, corals, oysters, finfish, humans) using microarray-based comparative genomic hybridization (CGH) and multilocus sequence analyses (MLSA).
PorA variable regions, FetA, and multilocus sequence typing analyses were performed according to standard procedures from the Neisseria Multi Locus Sequence Typing Web site (http://pubmlst.org/neisseria).org/neisseria
These trees were compared to published phylogenetic analyses for Neurospora based on multilocus sequence analysis (MLSA).
The relationships of these nine species have been established by phylogenetic analyses of multiple DNA sequences (multilocus sequence analysis, MLSA), which has provided a well supported tree spanning closely related Neurospora species and representatives of their neighboring genera [ 26- 28].
Microbial taxonomists later supplemented the analyses of phenotypic characters with chemotaxonomy, DNA DNA hybridization, analysis of 16S rRNA gene sequence, and multilocus sequence analysis (MLSA).
Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii.
