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Based on multilocus sequence analyses, however, these isolates are A. viridinutans, a mold phenotypically resembling but phylogenetically distinct from A. fumigatus.
Phylogenetic trees for these taxon sets, based on multilocus sequence analyses (MLSA; [ 26- 28]) are shown in Figure 1, with and without the reference taxon as indicated.
During the preparation of this manuscript a new L. taiwanensis species isolated from silage was described [ 39]. 16S rRNA encoding gene and multilocus sequence analyses showed that our strains isolated from rat intestine belong to L. taiwanensis species.
The relationships of V. cholerae have been studied in several ways, but the most useful insights have come from multilocus enzyme electrophoresis (12 ) and more recently by multilocus sequence analyses (4, 13, 14 ).
Subsequently, multilocus sequence analyses were performed and targeted the internal transcribed spacer (ITS) 1 and 2 regions flanking 5.8S rDNA, (partial) β-tubulin gene (benA), and (partial) calmodulin gene as previously described (1 – 4 ).
To begin to understand the underlying genetics that complicate species level discrimination, we compared the genomes of Harveyi clade members isolated from different environments (seawater, shrimp, corals, oysters, finfish, humans) using microarray-based comparative genomic hybridization (CGH) and multilocus sequence analyses (MLSA).
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PorA variable regions, FetA, and multilocus sequence typing analyses were performed according to standard procedures from the Neisseria Multi Locus Sequence Typing Web site (http://pubmlst.org/neisseria).org/neisseria
Relationships among these lineages are similar in the concatenated multilocus DNA sequence analyses of Yoon et al. (2006) and Verbruggen et al. (2010), but these relationships are poorly supported.
This clone is defined by multilocus sequence typing (MLST) analyses as ST-17, the so-called highly virulent clone (4 – 8 ).
Genetic diversity of PNSSP isolates was assessed using both pulsed-field gel electrophoresis (PFGE) and Multilocus Sequence Typing (MLST) analyses.
Microarray and multilocus sequence typing (MLST) analyses and surveys of biological diversity demonstrated the existence of several clonal lineages adapted to specific host plants inside the American phylotype II group of the R. solanacearum species complex [ 12, 14- 16].
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