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The sensitivity of the multilevel sectioning and immunohistochemistry was 75%; the negative predictive value was 95%.
Of those 11 lymph nodes, 1 harbored a micrometastasis, 5 harbored isolated tumor cells, and 5 remained negative after multilevel sectioning and IHC (Tables 2, 3).
Following the study protocol, lymph nodes >3 mm in greatest dimension were included into the study for OSNA analysis as well as multilevel sectioning and IHC.
However, 1 patient with a positive OSNA result and a negative initial H&E examination had a micrometastasis identified only after multilevel sectioning and IHC were performed.
The current study provides compelling evidence that OSNA is both very sensitive and specific for the assessment of lymph node metastases in patients with colon cancer and is comparable to extensive histopathologic workup using multilevel sectioning and IHC.
8, 9 Compared with standard H&E staining of lymph nodes in patients with colon cancer, it has been demonstrated that multilevel sectioning and the use of immunohistochemistry (IHC) can improve the detection of small tumor infiltrates in lymph nodes.
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The precise classification of pCR or ypT0 can be an effort- and time-consuming task provided that residual viable tumour cells could be identified in many cases upon rigorous microscopic examination (i.e., multilevel sectioning of the blocks containing the scarred area) [ 7, 121].
Indications of the Early Pleistocene paleopedogenesis were analyzed in multilevel sections of pedosediments at Early Acheulian sites within the Lori Plateau in Northern Armenia, with the purpose of reconstructing the paleoclimatic conditions during the initial stage of early human occupation of this region.
PDB wrote sections relating to the multilevel modelling and sample size components.
A multilevel model is established to simultaneously account for measurement correlation within the same test section and roughness evolution correlation within the same experimental site.
Multilevel regression models were used to examine variation in hospital rates of 'planned repeat caesarean section' and, among women who planned a vaginal birth, 'intrapartum caesarean section'.
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