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The biological significance of anti-GADPH must be related to the roles of GADPH as a multifunctional enzyme that is involved in apoptosis, oxidative stress, activation of transcription, membrane fusion, and vesicle transport.
Transglutaminase 2 (TG2) is a multifunctional enzyme that has guanine nucleotide binding and GTP hydrolyzing activity in addition to its transamidating function.
Fatty acid synthase (FASN), a multifunctional enzyme that is essential for the endogenous synthesis of long-chain fatty acids from its precursors acetyl-CoA and malonyl-CoA [31], has been recently shown to be a direct target of ΔNp63α [32].
Poly adenosine diphosphate-ribose polymerase-1 (PARP-1) is a multifunctional enzyme that is involved in two major cellular responses to oxidative and nitrosative (O/N) stress: detection and response to DNA damage via formation of protein-bound poly adenosine diphosphate-ribose (PAR), and formation of the soluble 2nd messenger monomeric adenosine diphosphate-ribose (mADPR).
The MFE2 gene, which encodes a multifunctional enzyme that catalyzes the second and third reactions in β-oxidation of fatty acids in peroxisomes, was necessary for full virulence although it was not required for the production of filaments during mating in vitro.
TGR is a multifunctional enzyme that can reduce glutathione disulfide and the disulfide bond of Trx.
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Eukaryotic fatty acid synthases (FASs) are huge multifunctional enzymes that carry out all enzymatic steps essential for fatty acid biosynthesis.
Bacterial type I polyketide synthases (PKSs) are complex, multifunctional enzymes that synthesize structurally diverse and medicinally important natural products.
KDO transferases are multifunctional enzymes that are able to transfer several KDO residues from CMP-KDO to different precursor molecules forming different linkages.
PARPs are multifunctional enzymes that play an important role in the repair of DNA single-strand breaks.
The GST proteins are a group of multifunctional enzymes that catalyze the conjunction and detoxification of xenobiotics during phase II enzyme detoxification [ 44].
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