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Then, the intensity of the fluorescence was examined with a multiwavelength multifunctional enzyme spectrometer (Synergy HT) and observed under a fluorescence microscope (Nikon 80i) (Yuan et al. 2014).
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However, depletion of either Phosphoenolpyruvate Carboxykinase (PEPCK) or Multifunctional enzyme type 2 (Mfe2) did block the growth of explanted brat tumors (Figs 2d and 3).
However, the multifunctional enzyme strategy is limited to small numbers of enzymes and restricted to suboptimal equimolar ratios of enzymes.
Common surface display systems that have been explored are cellulosomal (multi-enzyme) systems and multifunctional enzyme systems.
Different strategies are applied to produce multifunctional enzyme systems including enzyme cocktails, cellulosomes or xylanosomes and hybrid fusion enzymes (Conrado et al. 2008; Hu et al. 2013).
G-6-Pase is considered to be a multifunctional enzyme which is a characteristic constituent of the endoplasmic reticulum.
In some anaerobic thermophilic/thermotolerant microorganisms, few cellulases are organized as unique multifunctional enzyme complex, called the cellulosome.
The enzyme assemblies in multifunctional enzyme systems enable metabolic control and prevent metabolic cross-talk between competing pathways (Conrado et al. 2008).
Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) is a monomeric multifunctional enzyme that is expressed only in subanatomical portions of the brain, T lymphocytes, and postmeiotic male germ cells.
These strategies may be conducted separately or in combination, and they involve the following dominant microbial paradigms: cell-free enzyme systems, multi-enzyme (cellulosome) complexes, and multifunctional enzyme systems.
Human polymerase γ is a multifunctional enzyme with DNA polymerase, terminal transferase, 5′-deoxyribose phosphate (dRP) lyase and polynucleotide synthetase activities.
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