Hormone-sensitive lipase (HSL), a multifunctional enzyme exhibiting REH activity, has been demonstrated to be rate-limiting in the mobilization of adipose tissue RE stores [93].
(106) This transacetylase is a multifunctional enzyme with three catalytic activities (lysophospholipid transacetylase, sphingosine transacetylase, and acetylhydrolase) and its regulation differs for macrophages compared to monocytes.
The reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) is a multifunctional enzyme, possessing RNA-dependent DNA polymerase (RDDP) activity, DNA-dependent DNA polymerase (DDDP) activity and RNase H activity [ 1, 2].
It is not without precedent that multifunctional enzymes with overlapping activity 'fill holes' in fragmented essential metabolic pathways [ 25- 27].
It is a multifunctional enzyme with ATPase, endonuclease and 3′-phosphodiesterase activities; moreover, it binds to linear dsDNA, displays helicase activities and could thus be involved in DNA repair [8].
The number and position of CBMs in the multifunctional enzyme has an effect on the enzymatic activity of the given chimaera.
Vertebrate Hsd17β10 is a multifunctional enzyme that, in addition to a 17β-Hsd activity with sex steroids, acts as 3α-, 7β-, 7β-, 17β-, 20β-, and 21-oxidase of several different substrates (Shafqat et al. 2003).
Transglutaminase 2 (TG2) is a multifunctional enzyme that has guanine nucleotide binding and GTP hydrolyzing activity in addition to its transamidating function.
Other multifunctional enzyme chimeras have been created successfully and revealed promising combined activities [ 26- 28].
URA2 is a multifunctional enzyme catalyzing the first two enzymatic steps of the pathway, and its activity is negatively regulated in a feedback loop by the RNA precursor UTP, both at the gene expression level as well as at the enzymatic activity level [ 13, 17].
