This paper describes an approach to the problem of segmenting and classifying regions in multidimensional images that performs a joint two-step process.
Thankfully there are a number of Black women in media--Shonda Rhimes, Ava DuVernay, Oprah Winfrey, Kerry Washington, Mara Brock Akil, Issa Rae, and Viola Davis, just to name a few--who have taken it upon themselves to present broader, more complex, and more multidimensional images of Black women in media.
The software attribute isospots for each amastigotes and a line tracing of isospots displacement in multidimensional images.
Macrophage nuclei fluorescence signals allowed for automatic quantification of host cells in multidimensional images by the Imaris program.
The morphology of macrophage nuclei during L. amazonensis extrusion was followed in the multidimensional images of Hoechst staining; nuclei shrinkage and condensation of chromatin in the periphery of the nuclei were observed.
The quantification of amastigote transfer events by the observation of multidimensional images of infected BMDMØ-GFP treated with SLO revealed an increase in amastigote transfer events when compared with non-treated controls (Supplementary Fig. S1A and B, Video S5A).
After acquisition, images were processed by Imaris v.7.4.2 software (Bitplane AG, Andor Technology), which allowed for the construction of multidimensional images integrating spatial (tridimensional) and temporal data (acquisition of the same microscopic field in defined time intervals).
This is an open source image processing program designed for scientific multidimensional images.
Transfer of amastigotes between macrophages was counted by direct observation of multidimensional images.
Further experiments using multidimensional images and appropriate markers of T. cruzi differentiation will potentially reveal if differentiation into amastigotes takes place in PVs or in the cytosol and provide important information for future studies on drug delivery.
