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A multi-target method for the determination of 191 fungal metabolites in almonds, hazelnuts, peanuts and pistachios was developed.
This number was expected since a multi-target method covering a huge number of chemically diverse analytes always has to be a compromise.
Furthermore, it is the first time that a multi-target method is capable of determining all mycotoxins regulated in the European Union for maize [ 3– 6].
Based on an HPLC-MS/MS multi-target method developed previously by our group [ 4, 7], a new UHPLC-MS/MS method was developed on an Agilent 1290 Infinity UHPLC system coupled to a 6460 Triple Quadrupole mass spectrometer.
In 2008, Spanjer et al. [ 5] published the validation of an LC-MS/MS-based multi-target method for the determination of mycotoxins in various matrices, including peanuts (13 mycotoxins) and pistachios (24 mycotoxins).
This can be a problem for all multi-target methods and has to be assessed in further studies.
The exclusion of compounds for some assays regarding this problem could be beneficial for the performance of multi-target methods.
Still, such multi-target methods have to cope with huge differences in the relevant toxin concentrations.
A major advantage of LC-MS-based multi-target methods is the increased sample throughput compared to single-analyte methods.
Furthermore, none of the present multi-target methods using stable isotope dilution assays covers all mycotoxins regulated in maize.
A drawback of most multi-target methods is that they require extensive validation which is time- and cost-consuming and, hence, often reduced to a minimum.
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